![]() ![]() The purpose of rinsing the slide with water is to decolorize the slide. Once the heat fixing is done, the next step is to wash the slide using tap or distilled water, whichever is available. In other words, the heat acts as the mordant a substance used together with the dye to completely fix in a particular material. ![]() The heat is used to destroy the barrier and enabling the dye to interact with petodoglycan. For staining to be made possible, the barrier should be destroyed first. Note:Endospores have a permeable barrier, which prevents the dye from staining the cell’s structure. Examine the slide under the microscope.Rinse the slide and let it dry naturally.Counterstaining is done using safranin.You can use distilled water or tap water whichever is available. Remove the slide and let it cool for a few minutes.The purpose of this step is to steam the slide and not to overheat it. Keep the blotting paper moist by adding drops of malachite green. Heat, remove and re-heat the slide for about five minutes.Boiling water or a Bunsen burner can be used to heat the slide. ![]()
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